Comments on: What are the benefits of using RNAi to inhibit gene function over generating a gene knock-out? http://rnaimedicine.com/what-are-the-benefits-of-using-rnai-to-inhibit-gene-function-over-generating-a-gene-knock-out/ Sun, 25 Oct 2009 01:13:50 +0000 http://wordpress.org/?v=2.9.2 hourly 1 By: Genetic Engineering Center http://rnaimedicine.com/what-are-the-benefits-of-using-rnai-to-inhibit-gene-function-over-generating-a-gene-knock-out/comment-page-1/#comment-1 Genetic Engineering Center Fri, 08 May 2009 10:49:23 +0000 http://rnaimedicine.com/what-are-the-benefits-of-using-rnai-to-inhibit-gene-function-over-generating-a-gene-knock-out/#comment-1 <a href="http://GeneticTestingCenter.com">Genetic Testing Center</a> RNAi can be used for following purposes: 1. Immunity RNA interference is a vital part of the immune response to viruses and other foreign genetic material, especially in plants where it may also prevent self-propagation by transposons 2. Downregulation of genes Endogenously expressed miRNAs, including both intronic and intergenic miRNAs, are most important in translational repression and in the regulation of development, especially on the timing of morphogenesis and the maintenance of undifferentiated or incompletely differentiated cell types such as stem cells 3.Upregulation of genes RNA sequences (siRNA and miRNA) that are complementary to parts of a promoter can increase gene transcription, a phenomenon dubbed RNA activation. Part of the mechanism for how these RNA upregulate genes is known: dicer and argonaute are involved, and there is histone demethylation Whereas, knock out serves the only one purpose,i.e. elimination of a working gene! Well regarding problems, this technique is a bit tough to be used in laboratories. Genetic Testing Center

RNAi can be used for following purposes:
1. Immunity
RNA interference is a vital part of the immune response to viruses and other foreign genetic material, especially in plants where it may also prevent self-propagation by transposons
2. Downregulation of genes
Endogenously expressed miRNAs, including both intronic and intergenic miRNAs, are most important in translational repression and in the regulation of development, especially on the timing of morphogenesis and the maintenance of undifferentiated or incompletely differentiated cell types such as stem cells
3.Upregulation of genes
RNA sequences (siRNA and miRNA) that are complementary to parts of a promoter can increase gene transcription, a phenomenon dubbed RNA activation. Part of the mechanism for how these RNA upregulate genes is known: dicer and argonaute are involved, and there is histone demethylation

Whereas, knock out serves the only one purpose,i.e. elimination of a working gene!

Well regarding problems, this technique is a bit tough to be used in laboratories.

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